NQO-1 Antibody (A180) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80885

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Primate

Applications

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A180

Format

Azide and BSA Free

Concentration

1 mg/ml

View all NQO-1 Antibodies »

Product Summary for NQO-1 Antibody (A180) - Azide and BSA Free

Immunogen

Full length recombinant NQO1 from human lung [UniProt# P15559]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24475200)

Localization

Cytoplasmic (primarily), Nuclear (faintly)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NQO-1 Antibody (A180) - Azide and BSA Free

Western Blot: NQO-1 Antibody (A180)Azide and BSA Free [NBP2-80885]

Western Blot: NQO-1 Antibody (A180)Azide and BSA Free [NBP2-80885]

Western Blot: NQO-1 Antibody (A180) - Azide and BSA Free [NBP2-80885] - Detection of NQO1 in A549 lysates using NB 200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: NQO-1 Antibody (A180) - Azide and BSA Free [NBP2-80885]

Immunocytochemistry/ Immunofluorescence: NQO-1 Antibody (A180) - Azide and BSA Free [NBP2-80885]

Immunocytochemistry/Immunofluorescence: NQO-1 Antibody (A180) - Azide and BSA Free [NBP2-80885] - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) - Azide and BSA Free [NBP2-80885]

Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) - Azide and BSA Free [NBP2-80885]

Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) - Azide and BSA Free [NBP2-80885] - Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded human kidney tissue Image from the standard format of this antibody.
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Applications for NQO-1 Antibody (A180) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/ml

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:1000

Simple Western

1:100

Western Blot

1:1000
Application Notes
In Western blot a band can be seen at approx. 31 kDa representing NQO1. In ICC/IF, cytoplasmic staining was observed in U2OS cells. In IHC-P, staining was observed in the cytoplasm of human breast cancer tisSue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NQO-1

NAD(P)H:quinone oxidoreductase (NQO) is a ubiquitous flavoprotein enzyme which catalyzes the two-electron reduction/detoxification of quinones/quinones derivatives and it has two members - NQO1 (alias DT-diaphorase) which uses NADPH as a source of electrons, and NQO2 (NRH:quinone oxidoreductase 2) which employs dihydronicotinamide riboside (NRH) as electron donor. Expressed as cytosolic enzyme with maximum levels in liver, NQO1 is highly inducible by oxidants, electrophiles, phenolic compounds etc and its expression is regulated by Keap1/Nrf2/ARE pathway in mammals. NQO1 gene promotor contains multiple AREs (antioxidant response elements) and a functional XRE (xenobiotic response element), and AhR binding to XRE is critical for NQO1 induction by xenobiotics. Moreover, Nrf2/ARE and AhR/XRE have been proposed to coordinate in NQO1 expression regulation. Besides biotransformation, NQO1 also implicates in the maintenance of endogenous lipid-soluble antioxidants alpha-tocopherol and ubiquinone in their reduced/active forms, direct scavenging of superoxide anion radicals, stabilization of the tumor suppressor proteins including p53 and as a gatekeeper of the 20S proteasome pathway.

Long Name

NAD(P)H Dehydrogenase, Quinone 1

Alternate Names

Azoreductase, DHQU, DIA4, Diaphorase-4, Dioxin-inducible 1, DTD, Menadione Reductase, NMOR1, NQO1, QR1, Quinone Reductase 1

Gene Symbol

NQO1

Additional NQO-1 Products

Product Documents for NQO-1 Antibody (A180) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NQO-1 Antibody (A180) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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