NQO-2 Antibody (OTI3G4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73049

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all NQO-2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NQO2(NP_000895) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NQO-2 Antibody (OTI3G4) - Azide and BSA Free

Western Blot: NQO-2 Antibody (OTI3G4)Azide and BSA Free [NBP2-73049]

Western Blot: NQO-2 Antibody (OTI3G4)Azide and BSA Free [NBP2-73049]

Western Blot: NQO-2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73049] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NQO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NQO2.
Immunohistochemistry: NQO-2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73049]

Immunohistochemistry: NQO-2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73049]

Immunohistochemistry: NQO-2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73049] - Staining of paraffin-embedded Human pancreas tissue using anti-NQO2 mouse monoclonal antibody.
Western Blot: NQO-2 Antibody (OTI3G4)Azide and BSA Free [NBP2-73049]

Western Blot: NQO-2 Antibody (OTI3G4)Azide and BSA Free [NBP2-73049]

Western Blot: NQO-2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73049] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-NQO2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Applications for NQO-2 Antibody (OTI3G4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NQO-2

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis

Long Name

NAD(P)H Dehydrogenase, Quinone 2

Alternate Names

DHQV, DIA6, NMOR2, NQO2, QR2, Quinone Reductase 2

Gene Symbol

NQO2

Additional NQO-2 Products

Product Documents for NQO-2 Antibody (OTI3G4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NQO-2 Antibody (OTI3G4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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