NQO-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85221

Novus Biologicals, part of Bio-Techne
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NBP1-85221
NBP1-85221-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NQO-2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAGKKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNLEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NQO-2 Antibody

Western Blot: NQO-2 Antibody [NBP1-85221]

Western Blot: NQO-2 Antibody [NBP1-85221]

Western Blot: NQO-2 Antibody [NBP1-85221] - Analysis in control (vector only transfected HEK293T lysate) and NQO2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: NQO-2 Antibody [NBP1-85221]

Immunocytochemistry/ Immunofluorescence: NQO-2 Antibody [NBP1-85221]

Immunocytochemistry/Immunofluorescence: NQO-2 Antibody [NBP1-85221] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol.
Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85221]

Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85221]

Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85221] - Staining of human urinary bladder shows low expression as expected.
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Applications for NQO-2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000-1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NQO-2

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis

Long Name

NAD(P)H Dehydrogenase, Quinone 2

Alternate Names

DHQV, DIA6, NMOR2, NQO2, QR2, Quinone Reductase 2

Gene Symbol

NQO2

Additional NQO-2 Products

Product Documents for NQO-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NQO-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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