Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S364-51

Concentration

1 mg/ml

View all NrCAM Antibodies »

Product Summary for NrCAM Antibody (S364-51)

Immunogen

Fusion protein amino acids 30-845 (extracellular domain) of mouse NrCAM. Rat: 96% identity (795/822 amino acids identical). Human: 91% identity (753/822 amino acids identical) ~50% identity with Neurofascin.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Human and Rat.

Localization

Cell Membrane

Specificity

Approx 50% identity with NeurofascinDetects approx 160kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for NrCAM Antibody (S364-51)

Western Blot: NrCAM Antibody (S364-51) [NBP2-42191]

Western Blot: NrCAM Antibody (S364-51) [NBP2-42191]

Western Blot: NrCAM Antibody (S364-51) [NBP2-42191] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51 (NBP2-42191). Load: 10 ug. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody (NBP2-42191) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa.
Immunocytochemistry/ Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191]

Immunocytochemistry/ Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191]

Immunocytochemistry/Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NrCAM Monoclonal Antibody, Clone S364-51 (NBP2-42191). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody (NBP2-42191) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) NrCAM Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191]

Immunocytochemistry/ Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191]

Immunocytochemistry/Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.
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Applications for NrCAM Antibody (S364-51)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of NrCAM Antibody was sufficient for detection of NrCAM in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NrCAM

NRCAM - neuronal cell adhesion molecule

Long Name

Neuronal Cell Adhesion Molecule

Alternate Names

Bravo

Gene Symbol

NRCAM

UniProt

Q810U4

Additional NrCAM Products

Product Documents for NrCAM Antibody (S364-51)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NrCAM Antibody (S364-51)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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