Nrf1 Antibody (S02-9A7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14960

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14960-100ul
NBP3-14960-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-9A7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nrf1 Antibodies »

Product Summary for Nrf1 Antibody (S02-9A7)

Immunogen

A synthetic peptide of human Nrf1 (Uniprot#: Q16656)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nrf1 Antibody (S02-9A7)

Western Blot: Nrf1 Antibody (S02-9A7) [NBP3-14960]

Western Blot: Nrf1 Antibody (S02-9A7) [NBP3-14960]

Western Blot: Nrf1 Antibody (S02-9A7) [NBP3-14960] - Western blot detection of Nrf1 in C6, 3T3, Hela cell lysates using Nrf1 Rabbit mAb (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 68kDa.
Immunocytochemistry/ Immunofluorescence: Nrf1 Antibody (S02-9A7) [NBP3-14960]

Immunocytochemistry/ Immunofluorescence: Nrf1 Antibody (S02-9A7) [NBP3-14960]

Immunocytochemistry/Immunofluorescence: Nrf1 Antibody (S02-9A7) [NBP3-14960] - Immunofluorescence of Nrf1 (green) in Hela cells using Nrf1 Rabbit mAb at dilution 1/200, and DAPI (blue)
Immunohistochemistry-Paraffin: Nrf1 Antibody (S02-9A7) [NBP3-14960]

Immunohistochemistry-Paraffin: Nrf1 Antibody (S02-9A7) [NBP3-14960]

Immunohistochemistry-Paraffin: Nrf1 Antibody (S02-9A7) [NBP3-14960] - Immunohistochemistry of Nrf1 in paraffin-embedded Human colon cancer tissue using Nrf1 Rabbit mAb at dilution 1/5

Applications for Nrf1 Antibody (S02-9A7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20

Immunohistochemistry

1/20

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1/2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nrf1

NRF1 encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Long Name

NF-E2-related Factor 1

Alternate Names

HBZ17, LCR-F1, NFE2L1, TCF11

Gene Symbol

NRF1

Additional Nrf1 Products

Product Documents for Nrf1 Antibody (S02-9A7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nrf1 Antibody (S02-9A7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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