NRF3 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93557
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 330-550 of human NRF3 (NP_004280.5). DPTARTSQSQEPFLQLNSHTTNPEQTLPGTNLTGFLSPVDNHMRNLTSQDLLYDLDINIFDEINLMSLATEDNFDPIDVSQLFDEPDSDSGLSLDSSHNNTSVIKSNSSHSVCDEGAIGYCTDHESSSHHDLEGAVGGYYPEPSKLCHLDQSDSDFHGDLTFQHVFHNHTYHLQPTAPESTSEPFPWPGKSQKIRSRYLEDTDRNLSRDEQRAKALHIPFS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NRF3 Antibody - Azide and BSA Free
![Western Blot: NRF3 AntibodyAzide and BSA Free [NBP2-93557]](https://resources.bio-techne.com/images/products/NRF3 Antibody-Western Blot-NBP2-93557-img0001.jpg "Western Blot: NRF3 AntibodyAzide and BSA Free [NBP2-93557]")
Western Blot: NRF3 AntibodyAzide and BSA Free [NBP2-93557]
Western Blot: NRF3 Antibody [NBP2-93557] - Analysis of extracts of various cell lines, using NRF3 antibody (NBP2-93557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin-NRF3 Antibody [NBP2-93557] -
Immunohistochemistry-Paraffin-NRF3 Antibody [NBP2-93557] -Analysis of NFE2L3 in paraffin-embedded rat colon tissue using NFE2L3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry-Paraffin-NRF3 Antibody [NBP2-93557] -
Immunohistochemistry-Paraffin-NRF3 Antibody [NBP2-93557] -Analysis of NFE2L3 in paraffin-embedded human colon carcinoma tissue using NFE2L3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.Applications for NRF3 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NRF3
Alternate Names
NF-E2-related factor 3, NFE2-related factor 3, Nrf3, NRF3nuclear factor erythroid 2-related factor 3, nuclear factor (erythroid-derived 2)-like 3, Nuclear factor, erythroid derived 2, like 3, nuclear factor-erythroid 2 p45-related factor 3, nuclear factor-erythroid 2-related factor 3
Gene Symbol
NFE2L3
Additional NRF3 Products
Product Documents for NRF3 Antibody - Azide and BSA Free
Product Specific Notices for NRF3 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov