NRSF Antibody (8R8Z4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16128

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16128-100ul
NBP3-16128-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8R8Z4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all REST/NRSF Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human NRSF (Q13127). KGEPHGLENMELRSLELSVVEPQPVFEASGAPDIYSSNKDLPPETPGAEDKGKSSKTKPFRCKPCQYEAESEEQFVHHIRVHSAKKFFVEESAEKQAKARE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NRSF Antibody (8R8Z4)

Western Blot: NRSF Antibody (8R8Z4) [NBP3-16128]

Western Blot: NRSF Antibody (8R8Z4) [NBP3-16128]

Western Blot: NRSF Antibody (8R8Z4) [NBP3-16128] - Western blot analysis of extracts of Rat brain, using NRSF Rabbit mAb (NBP3-16128) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western Blot: NRSF Antibody (8R8Z4) [NBP3-16128]

Western Blot: NRSF Antibody (8R8Z4) [NBP3-16128]

Western Blot: NRSF Antibody (8R8Z4) [NBP3-16128] - Western blot analysis of extracts of various cell lines, using NRSF Rabbit mAb (NBP3-16128) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation: NRSF Antibody (8R8Z4) [NBP3-16128]

Immunoprecipitation: NRSF Antibody (8R8Z4) [NBP3-16128]

Immunoprecipitation: NRSF Antibody (8R8Z4) [NBP3-16128] - Immunoprecipitation analysis of 300ug extracts of U-87MG cells using 3ug NRSF antibody (NBP3-16128). Western blot was performed from the immunoprecipitate using NRSF antibody (NBP3-16128) at a dilition of 1:1000.

Applications for NRSF Antibody (8R8Z4)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: REST/NRSF

REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.

Long Name

RE1-Silencing Transcription Factor

Alternate Names

NRSF, XBR

Gene Symbol

REST

Additional REST/NRSF Products

Product Documents for NRSF Antibody (8R8Z4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NRSF Antibody (8R8Z4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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