NSDHL Antibody (20F2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26457
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 20F2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human NSDHL [UniProt Q15738]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NSDHL Antibody (20F2)
Immunohistochemistry: NSDHL Antibody (20F2) [NBP3-26457] -
Immunohistochemistry: NSDHL Antibody (20F2) [NBP3-26457] - Image of NSDHL Antibody (20F2) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot: NSDHL Antibody (20F2) [NBP3-26457] -
Western Blot: NSDHL Antibody (20F2) [NBP3-26457] - Positive Western Blot detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, HEK293 whole cell lysate.All lanes: NSDHL Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Applications for NSDHL Antibody (20F2)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: NSDHL
Alternate Names
EC 1.1.1.170, H105e3, member 1, NAD(P) dependent steroid dehydrogenase-like, SDR31E1, sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, XAP104
Gene Symbol
NSDHL
Additional NSDHL Products
Product Documents for NSDHL Antibody (20F2)
Product Specific Notices for NSDHL Antibody (20F2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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