NSP 5 alpha 3 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-517

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all NSP 5 alpha 3 alpha Antibodies »

Product Specifications

Immunogen

An internal synthetic peptide made to the human NSP 5 alpha 3 alpha protein sequence (between residues 1-100). [UniProt# Q5M775]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: mouse (83%), rat (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NSP 5 alpha 3 alpha Antibody

Immunocytochemistry/ Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517]

Immunocytochemistry/ Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517]

Immunocytochemistry/Immunofluorescence: NSP 5 alpha 3 alpha Antibody [NB100-517] - Immunostaining of HN30 and MCF-12a cells. MCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (NSP 5a3a-FITC): non-treated MCF-12a FITC stained for NSP 5a3a, MCF-12a pNSP 5a3a (DAPI): HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (NSP 5a3a-FITC): non-treated HN30 FITC stained for NSP 5a3a, HN30 pNSP 5a3a (DAPI): White arrows indicate apoptotic bodies. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/article/306/text/) licensed under a CC-BY license.
Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517]

Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517]

Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Western Blot analysis of total lysates from asynchronous HN30 cells and MCF-12a 3 days post-transfection. NT: non-treated, OB: only buffer, EGFP: only pcDNA3.1/CT-GFP vector, NSP 5a3a/EGFP: pcDNA 3.1/CT-GFP and pcDNA 3.0 NSP 5a3a. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/article/306/text/) licensed under a CC-BY license.
Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517]

Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517]

Western Blot: NSP 5 alpha 3 alpha Antibody [NB100-517] - Detection of NSP 5 alpha 3 alpha in HeLa whole cell lysate (RIPA) using NB100-517. 0.5ug/ml dilution, 15 second ECL detection.
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Applications for NSP 5 alpha 3 alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250

Western Blot

1:500-1:2000
Application Notes
In Western blot, a band is seen at ~80 kDa. A second band may be seen at ~60 kDa. The specificity of this band is not known.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NSP 5 alpha 3 alpha

Nuclear structure protein 5 (NSP5) or NSP 5 alpha 3 alpha (NSP 5a3a) is a novel structural protein which has been found to be over-expressed differently in certain cancer cell lines such as: HT-29, MCF-7, Hela, HN30, Saos-2, CEM, and H23, however, it is barely detectable in normal body tissues except for testis wherein NSP 5a3a is highly expressed. NSP 5a3a interacts with cytoplasmic - nuclear proteins B23 which is known to implicate in multiple cellular processes such as mitosis, cell growth control, and apoptosis, ribosome biogenesis, and rRNA transcription/processing. NSP 5a3a also interacts with hnRNP-L which plays a role in RNA metabolism and rRNA processing. NSP proteins (NSP 5b3a, 5a3b, and 5b3b) have been implicated in several cellular activities such as intracellular trafficking, cellular/subcellular integrity, protein-protein interactions with other structural proteins as well as in protein complex stabilization and scaffolding thus facilitating homo or hetero dimerization of protein complexes. Over-expression of NSP 5a3a has been shown to induce apoptosis in head and neck carcinoma cell lines in particular in HN30 being p53 independent yet involving a novel p73 dependent mechanism wherein NSP 5a3a induces apoptosis through p73-DAXX and TRAF2-TRADD.

Alternate Names

cytospin-B, CYTSBcytospin B, FLJ36955, HCMOGT-1, NSP, NSP5, Nuclear structure protein 5, sperm antigen HCMOGT 1, Sperm antigen HCMOGT-1, sperm antigen with calponin homology and coiled coil domains 1, sperm antigen with calponin homology and coiled-coil domains 1cytokinesis and spindle organization B, sperm antigen with calponin-like and coiled coil domains 1, structure protein NSP5a3a, structure protein NSP5a3b, structure protein NSP5b3a, structure protein NSP5b3b

Entrez Gene IDs

92521 (Human)

Gene Symbol

SPECC1

UniProt

Q5M775 (Human)

Additional NSP 5 alpha 3 alpha Products

Product Documents for NSP 5 alpha 3 alpha Antibody

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Certificate of Analysis

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Product Specific Notices for NSP 5 alpha 3 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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