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NSUN4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19594

Novus Biologicals, part of Bio-Techne
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NBP2-19594

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NSUN4. The exact sequence is proprietary.

Reactivity Notes

Bovine (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NSUN4 Antibody

Western Blot: NSUN4 Antibody [NBP2-19594]

Western Blot: NSUN4 Antibody [NBP2-19594]

Western Blot: NSUN4 Antibody [NBP2-19594] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NSUN4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody
Immunohistochemistry-Paraffin: NSUN4 Antibody [NBP2-19594]

Immunohistochemistry-Paraffin: NSUN4 Antibody [NBP2-19594]

Immunohistochemistry-Paraffin: NSUN4 Antibody [NBP2-19594] - Human colon carcinoma, using NSUN4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: NSUN4 Antibody [NBP2-19594]

Western Blot: NSUN4 Antibody [NBP2-19594]

Western Blot: NSUN4 Antibody [NBP2-19594] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.

Applications for NSUN4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NSUN4

May have S-adenosyl-L-methionine-dependent methyl-transferase activity.

Alternate Names

EC 2.1.1, EC 2.1.1.-, EC 2.1.1.29, MGC22960, NOL1/NOP2/Sun domain family 4, NOL1/NOP2/Sun domain family member 4, NOL1/NOP2/Sun domain family, member 4, NOP2/Sun domain family, member 4, putative methyltransferase NSUN4, RP4-603I14.2

Gene Symbol

NSUN4

UniProt

Additional NSUN4 Products

Product Documents for NSUN4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NSUN4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.