NSUN5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89416
![Western Blot: NSUN5 Antibody [NBP1-89416]](https://resources.bio-techne.com/images/products/NSUN5-Antibody-Western-Blot-NBP1-89416-img0008.jpg "Western Blot: NSUN5 Antibody [NBP1-89416]")
![Western Blot: NSUN5 Antibody [NBP1-89416]](https://resources.bio-techne.com/images/products/NSUN5-Antibody-Western-Blot-NBP1-89416-img0009.jpg "Western Blot: NSUN5 Antibody [NBP1-89416]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QLPRFVRVNTLKTCSDDVVDYFKRQGFSYQGRASSLDDLRALKGKHFLLDPLMPELLVFPAQTDLHEHPLYRAGHLIL
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NSUN5 Antibody
![Immunocytochemistry/ Immunofluorescence: NSUN5 Antibody [NBP1-89416]](https://resources.bio-techne.com/images/products/NSUN5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89416-img0004.jpg "Immunocytochemistry/ Immunofluorescence: NSUN5 Antibody [NBP1-89416]")
Immunocytochemistry/ Immunofluorescence: NSUN5 Antibody [NBP1-89416]
Immunocytochemistry/Immunofluorescence: NSUN5 Antibody [NBP1-89416] - Staining of human cell line A-431 shows positivity in nucleus and nucleoli. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: NSUN5 Antibody [NBP1-89416]](https://resources.bio-techne.com/images/products/NSUN5-Antibody-Immunohistochemistry-Paraffin-NBP1-89416-img0006.jpg "Immunohistochemistry-Paraffin: NSUN5 Antibody [NBP1-89416]")
Immunohistochemistry-Paraffin: NSUN5 Antibody [NBP1-89416]
Immunohistochemistry-Paraffin: NSUN5 Antibody [NBP1-89416] - Staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.Applications for NSUN5 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NSUN5
Alternate Names
EC 2.1.1.-, FLJ10267, member 5A, MGC986, NOL1, NOL1/NOP2/Sun domain family member 5, NOL1/NOP2/Sun domain family, member 5, NOL1R(NOL1), NOL1-related protein, NOP2/Sun domain family, member 5, NSUN5A, p120, putative methyltransferase NSUN5, WBSCR20, WBSCR20A, Williams-Beuren syndrome chromosomal region 20A protein, Williams-Beuren syndrome critical region protein 20 copy A, Ynl022cL
Gene Symbol
NSUN5
Additional NSUN5 Products
Product Documents for NSUN5 Antibody
Product Specific Notices for NSUN5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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