NSUN6 Antibody (PSH02-80)

Western Blot: NSUN6 Antibody (PSH02-80) [NBP3-32667]

Western Blot: NSUN6 Antibody (PSH02-80) [NBP3-32667] - Western blot analysis of NSUN6 on different lysates with Rabbit anti-NSUN6 antibody (NBP3-32667) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: Rat pancreas tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 52 kDa
Observed band size: 52 kDa

Exposure time: 1 minute 58 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32667) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: NSUN6 Antibody (PSH02-80) [NBP3-32667]

Immunocytochemistry/ Immunofluorescence: NSUN6 Antibody (PSH02-80) [NBP3-32667] - Immunocytochemistry analysis of HeLa cells labeling NSUN6 with Rabbit anti-NSUN6 antibody (NBP3-32667) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NSUN6 antibody (NBP3-32667) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: NSUN6 Antibody (PSH02-80) [NBP3-32667]

Flow Cytometry: NSUN6 Antibody (PSH02-80) [NBP3-32667] - Flow cytometric analysis of HeLa cells labeling NSUN6.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32667, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).