Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 11D1cc

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NT-proBNP Antibodies »

Product Summary for NT-proBNP Antibody (11D1cc)

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic NT-proBNP, corresponding to a.a.r. 28-45, conjugated with carrier protein.

Epitope

a.a.r. 31-39

Localization

Secreted

Specificity

Human NT-proBNP and proBNP.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NT-proBNP Antibody (11D1cc)

Western Blot: NT-proBNP Antibody (11D1cc) [NB120-13059]

Western Blot: NT-proBNP Antibody (11D1cc) [NB120-13059]

Western Blot: proBNP Antibody (11D1) [NB120-13059] - Detection of human recombinant proBNP expressed in mammalian cells in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Lanes: 1 - MAb 5B61-12; 2 - 29D125-12; 3 - 15F1113-24; 4 - 13G1213-20; 5 - 18H513-20; 6 - 16F313-20; 7 - 11D131-39; 8 - 15D748-56; 9 - 15C463-71; 10 - 24E1167-76. Antigen: recombinant proBNP expressed in mammalian cells 0.15 ug/line. MAbs 11D131-39, 15D748-56, 15C463-71 and 24E1167-76 does not recognize glycosylated proBNP expressed in the mammalian cell line.
Western Blot: NT-proBNP Antibody (11D1cc) [NB120-13059]

Western Blot: NT-proBNP Antibody (11D1cc) [NB120-13059]

Western Blot: proBNP Antibody (11D1) [NB120-13059] - Tricine-SDS-PAGE in reducing conditions of recombinant NT-proBNP and proBNP expressed in E. coli. Lane 1: low molecular weight standards (Bio-Rad) Lane 2: proBNP, 3 ug Lane 3: NT-proBNP, 3 ug Gel staining: Coomassie brilliant blue R-250.
Western Blot: NT-proBNP Antibody (11D1cc) [NB120-13059]

Western Blot: NT-proBNP Antibody (11D1cc) [NB120-13059]

Western Blot: proBNP Antibody (11D1) [NB120-13059] - Detection of human recombinant NT-proBNP expressed in E. coli in Western blotting by different monoclonal antibodies after Tricine- SDS gel electrophoresis. Lanes: 1 - MAb 5B61-12; 2 - 15F1113-24; 3 - 11D131-39; 4 - 15D748-56; 5 - 24E1167-76. Antigen: recombinant NT-proBNP expressed in E. coli, 2.5 ug/well.

Applications for NT-proBNP Antibody (11D1cc)

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NT-proBNP

Alternate Names

BNP, brain type natriuretic peptide, Gamma-brain natriuretic peptide, natriuretic peptide precursor B, natriuretic peptides B, natriuretic protein

Entrez Gene IDs

4879 (Human)

Gene Symbol

NPPB

UniProt

P16860

Additional NT-proBNP Products

Product Documents for NT-proBNP Antibody (11D1cc)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NT-proBNP Antibody (11D1cc)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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