Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 13G12cc

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NT-proBNP Antibodies »

Product Summary for NT-proBNP Antibody (13G12cc)

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic NT-proBNP, corresponding to a.a.r. 13-27, conjugated with carrier protein.

Epitope

a.a.r. 15-20

Localization

Secreted

Specificity

Human NT-proBNP and proBNP

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for NT-proBNP Antibody (13G12cc)

Western Blot: NT-proBNP Antibody (13G12cc) [NB120-14712]

Western Blot: NT-proBNP Antibody (13G12cc) [NB120-14712]

Western Blot: NT-proBNP Antibody (13G12) [NB120-14712] - Tricine-SDS-PAGE in reducing conditions of recombinant NT-proBNP and proBNP expressed in E. coli. Lane 1: low molecular weight standards (Bio-Rad) Lane 2: proBNP, 3 ug Lane 3: NT-proBNP, 3 ug Gel staining: Coomassie brilliant blue R-250
ELISA: NT-proBNP Antibody (13G12cc) [NB120-14712]

ELISA: NT-proBNP Antibody (13G12cc) [NB120-14712]

ELISA: NT-proBNP Antibody (13G12) [NB120-14712] - Calibration curve for NT-proBNP 15C4-13G12 assay using NT-proBNP Antibody as a detection antibody: 13G1213-20 (Eu3+ - labeled) and biotinylated capture antibody. Antigen: human recombinant NT-proBNP expressed in E. coli
Sandwich ELISA: NT-proBNP Antibody (13G12) [NB120-14712] - Stability studies of endogenous NT-proBNP being measured in sandwich immunoassay.

Applications for NT-proBNP Antibody (13G12cc)

Application
Recommended Usage

ELISA

1:100-1:2000

Sandwich ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NT-proBNP

Alternate Names

BNP, brain type natriuretic peptide, Gamma-brain natriuretic peptide, natriuretic peptide precursor B, natriuretic peptides B, natriuretic protein

Entrez Gene IDs

4879 (Human)

Gene Symbol

NPPB

Additional NT-proBNP Products

Product Documents for NT-proBNP Antibody (13G12cc)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NT-proBNP Antibody (13G12cc)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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