NT-proBNP Antibody (24E11cc)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-13123
Key Product Details
Species Reactivity
Validated:
Human
Applications
Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 24E11cc
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for NT-proBNP Antibody (24E11cc)
Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic NT-proBNP, corresponding to a.a.r. 61-76, conjugated with carrier protein.
Epitope
a.a.r. 67-76
Localization
Secreted
Specificity
Human NT-proBNP and proBNP
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for NT-proBNP Antibody (24E11cc)
![Western Blot: NT-proBNP Antibody (24E11cc) [NB120-13123]](https://resources.bio-techne.com/images/products/proBNP-Antibody-24E11-Western-Blot-NB120-13123-img0009.jpg "Western Blot: NT-proBNP Antibody (24E11cc) [NB120-13123]")
Western Blot: NT-proBNP Antibody (24E11cc) [NB120-13123]
Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Detection of human recombinant NT-proBNP expressed in E. coli in Western blotting by different monoclonal antibodies after Tricine- SDS gel electrophoresis. Lanes: 1 - MAb 5B61-12; 2 - 15F1113-24; 3 - 11D131-39; 4 - 15D748-56; 5 - 24E1167-76. Antigen: recombinant NT-proBNP expressed in E. coli, 2.5 ug/well.![Western Blot: NT-proBNP Antibody (24E11cc) [NB120-13123]](https://resources.bio-techne.com/images/products/proBNP-Antibody-24E11-Western-Blot-NB120-13123-img0011.jpg "Western Blot: NT-proBNP Antibody (24E11cc) [NB120-13123]")
Western Blot: NT-proBNP Antibody (24E11cc) [NB120-13123]
Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Tricine-SDS-PAGE in reducing conditions of recombinant NT-proBNP and proBNP expressed in E. coli. Lane 1: low molecular weight standards. Lane 2: proBNP, 3 ug Lane 3: NT-proBNP, 3 ug Gel staining: Coomassie brilliant blue R-250.![Western Blot: NT-proBNP Antibody (24E11cc) [NB120-13123]](https://resources.bio-techne.com/images/products/proBNP-Antibody-24E11-Western-Blot-NB120-13123-img0010.jpg "Western Blot: NT-proBNP Antibody (24E11cc) [NB120-13123]")
Western Blot: NT-proBNP Antibody (24E11cc) [NB120-13123]
Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Detection of human recombinant proBNP expressed in mammalian cells in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Antigen: recombinant proBNP expressed in mammalian cells 0.15 ug/line. MAbs 7, 8, 9 and 10 do not recognize glycosylated proBNP expressed in the mammalian cell line.Applications for NT-proBNP Antibody (24E11cc)
Application
Recommended Usage
Sandwich ELISA
1:100-1:2000
Western Blot
1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NT-proBNP
Alternate Names
BNP, brain type natriuretic peptide, Gamma-brain natriuretic peptide, natriuretic peptide precursor B, natriuretic peptides B, natriuretic protein
Entrez Gene IDs
4879 (Human)
Gene Symbol
NPPB
Additional NT-proBNP Products
Product Documents for NT-proBNP Antibody (24E11cc)
Product Specific Notices for NT-proBNP Antibody (24E11cc)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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