NT2NL Antibody (2G12-2A5)
Novus Biologicals, part of Bio-Techne | Catalog # H00388677-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2G12-2A5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NOTCH2NL (AAH19835, 1 a.a. ~ 236 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGICLNLPGSYQCQCLQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPETVRRGTELWERDREVWNGKEHDEN
Reactivity Notes
Other species not tested.
Specificity
NOTCH2NL - Notch homolog 2 (Drosophila) N-terminal like
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for NT2NL Antibody (2G12-2A5)
![Western Blot: NT2NL Antibody (2G12-2A5) [H00388677-M01]](https://resources.bio-techne.com/images/products/NT2NL-Antibody-2G12-2A5-Western-Blot-H00388677-M01-img0007.jpg "Western Blot: NT2NL Antibody (2G12-2A5) [H00388677-M01]")
Western Blot: NT2NL Antibody (2G12-2A5) [H00388677-M01]
Western Blot: NT2NL Antibody (2G12-2A5) [H00388677-M01] - NOTCH2NL monoclonal antibody (M01), clone 2G12-2A5 Analysis of NOTCH2NL expression in HL-60.![Immunocytochemistry/ Immunofluorescence: NT2NL Antibody (2G12-2A5) [H00388677-M01]](https://resources.bio-techne.com/images/products/NT2NL-Antibody-2G12-2A5-Immunocytochemistry-Immunofluorescence-H00388677-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: NT2NL Antibody (2G12-2A5) [H00388677-M01]")
Immunocytochemistry/ Immunofluorescence: NT2NL Antibody (2G12-2A5) [H00388677-M01]
Immunocytochemistry/Immunofluorescence: NT2NL Antibody (2G12-2A5) [H00388677-M01] - Analysis of monoclonal antibody to NOTCH2NL on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: NT2NL Antibody (2G12-2A5) [H00388677-M01] - Detection limit for recombinant GST tagged NOTCH2NL is approximately 0.1ng/ml as a capture antibody.
Applications for NT2NL Antibody (2G12-2A5)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NT2NL
Alternate Names
FLJ35032, FLJ55807, N2NNotch homolog 2 (Drosophila) N-terminal like, notch 2 N-terminal like, Notch homolog 2 N-terminal like protein, notch homolog 2 N-terminal-like protein
Entrez Gene IDs
388677 (Human)
Gene Symbol
NOTCH2NLA
UniProt
Additional NT2NL Products
Product Documents for NT2NL Antibody (2G12-2A5)
Product Specific Notices for NT2NL Antibody (2G12-2A5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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