NT5C3 Antibody (009)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90252
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 009
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human NT5C3 (Accession#: NP_001002010; Met12-Leu297).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for NT5C3 Antibody (009)
Western Blot: NT5C3 Antibody (009) [NBP2-90252]
Western Blot: NT5C3 Antibody (009) [NBP2-90252] - Anti-NT5C3 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:38 kDa. Observed band size:39 kDa.Immunoprecipitation: NT5C3 Antibody (009) [NBP2-90252]
Immunoprecipitation: NT5C3 Antibody (009) [NBP2-90252] - NT5C3 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate. Lane B:0.5 mg Jurkat Whole Cell Lysate 0.5 uL anti-NT5C3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NT5C3 rabbit monoclonal antibody,at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa.Applications for NT5C3 Antibody (009)
Application
Recommended Usage
Immunoprecipitation
1-2 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NT5C3
Alternate Names
5'-nucleotidase, cytosolic III, cN-IIIMGC87828, cytosolic 5'-nucleotidase 3, Cytosolic 5'-nucleotidase III, p36, P5N1, P5N-1, P5'N-1MGC27337, PN-IMGC87109, PSN1UMPH, Pyrimidine 5'-nucleotidase 1, UMPH1EC 3.1.3.5, uridine 5' monophosphate hydrolase 1, Uridine 5'-monophosphate hydrolase 1
Gene Symbol
NT5C3A
Additional NT5C3 Products
Product Documents for NT5C3 Antibody (009)
Product Specific Notices for NT5C3 Antibody (009)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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