Nuclear Factor Erythroid Derived 2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82580
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]](https://resources.bio-techne.com/images/products/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0014.jpg "Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]")
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]](https://resources.bio-techne.com/images/products/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0017.jpg "Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPES
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (85%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Nuclear Factor Erythroid Derived 2 Antibody
![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]](https://resources.bio-techne.com/images/products/Nuclear-Factor-Erythroid-Derived-2-Antibody-Western-Blot-NBP1-82580-img0019.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]")
Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]
Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Analysis in control (vector only transfected HEK293T lysate) and NFE2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]](https://resources.bio-techne.com/images/products/Nuclear-Factor-Erythroid-Derived-2-Antibody-Western-Blot-NBP1-82580-img0018.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]")
Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]
Western Blot: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580] - Analysis in human cell line HEL.![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]](https://resources.bio-techne.com/images/products/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0015.jpg "Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]")
![Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]](https://resources.bio-techne.com/images/products/Nuclear-Factor-Erythroid-Derived-2-Antibody-Immunohistochemistry-Paraffin-NBP1-82580-img0016.jpg "Immunohistochemistry-Paraffin: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]")
![Simple Western: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]](https://resources.bio-techne.com/images/products/Nuclear-Factor-Erythroid-Derived-2-Antibody-Simple-Western-NBP1-82580-img0006.jpg "Simple Western: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]")
![Simple Western: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]](https://resources.bio-techne.com/images/products/Nuclear-Factor-Erythroid-Derived-2-Antibody-Simple-Western-NBP1-82580-img0007.jpg "Simple Western: Nuclear Factor Erythroid Derived 2 Antibody [NBP1-82580]")
Applications for Nuclear Factor Erythroid Derived 2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50-1:200
Simple Western
1:20
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. br/>In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nuclear Factor Erythroid Derived 2
Alternate Names
Leucine zipper protein NF-E2, NF-E2, nuclear factor (erythroid-derived 2), 45kD, nuclear factor (erythroid-derived 2), 45kDa, Nuclear factor, erythroid-derived 2 45 kDa subunit, p45, p45 NF-E2, transcription factor NF-E2 45 kDa subunit
Gene Symbol
NFE2
Additional Nuclear Factor Erythroid Derived 2 Products
Product Documents for Nuclear Factor Erythroid Derived 2 Antibody
Product Specific Notices for Nuclear Factor Erythroid Derived 2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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