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Nucleolin Antibody (S08-6H3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19897

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-19897-100ul
NBP3-19897-50ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-6H3

Concentration

0.3 mg/ml

Product Summary for Nucleolin Antibody (S08-6H3)

Immunogen

A synthetic peptide of human Nucleolin (Uniprot # P19338)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nucleolin Antibody (S08-6H3)

Western Blot: Nucleolin Antibody (S08-6H3) [NBP3-19897]

Western Blot: Nucleolin Antibody (S08-6H3) [NBP3-19897]

Western Blot: Nucleolin Antibody (S08-6H3) [NBP3-19897] - Western blot detection of Nucleolin in Hela, A549, HL-60 using NBP3-19897 (1:1000 diluted)

Applications for Nucleolin Antibody (S08-6H3)

Application
Recommended Usage

Western Blot

1/2000-1/10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nucleolin

Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. Nucleolin antibodies are commonly used as a mammalian nucleolus marker.

Alternate Names

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Gene Symbol

NCL

Additional Nucleolin Products

Product Documents for Nucleolin Antibody (S08-6H3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nucleolin Antibody (S08-6H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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