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Nudel Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97160

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human Nudel fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Nudel Antibody

Western Blot: Nudel Antibody [NBP2-97160]

Western Blot: Nudel Antibody [NBP2-97160]

Western Blot: Nudel Antibody [NBP2-97160] - Anti-Nudel rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 43 kDa
Immunoprecipitation: Nudel Antibody [NBP2-97160]

Immunoprecipitation: Nudel Antibody [NBP2-97160]

Immunoprecipitation: Nudel Antibody [NBP2-97160] - Nudel was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-Nudel rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Nudel rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 43 kDa.

Applications for Nudel Antibody

Application
Recommended Usage

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nudel

Nudel encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

DKFZp451M0318, EOPA, MITAP1mitosin-associated protein MITAP1, Mitosin-associated protein 1, nuclear distribution protein nudE-like 1, nudE nuclear distribution gene E homolog (A. nidulans)-like 1, nudE nuclear distribution gene E homolog like 1, nudE nuclear distribution gene E homolog like 1 (A. nidulans), NUDELendooligopeptidase A, NUDE-like protein, Protein Nudel

Gene Symbol

NDEL1

Additional Nudel Products

Product Documents for Nudel Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nudel Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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