Nuf2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31632
![Immunocytochemistry/ Immunofluorescence: Nuf2 Antibody [NBP1-31632]](https://resources.bio-techne.com/images/products/Nuf2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31632-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Nuf2 Antibody [NBP1-31632]")
![Immunocytochemistry/ Immunofluorescence: Nuf2 Antibody [NBP1-31632]](https://resources.bio-techne.com/images/products/Nuf2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31632-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Nuf2 Antibody [NBP1-31632]")
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein corresponding to human NUF2.
Localization
Nucleus, Kinetochore
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Nuf2 Antibody
![Western Blot: Nuf2 Antibody [NBP1-31632]](https://resources.bio-techne.com/images/products/Nuf2-Antibody-Western-Blot-NBP1-31632-img0009.jpg "Western Blot: Nuf2 Antibody [NBP1-31632]")
Western Blot: Nuf2 Antibody [NBP1-31632]
Western Blot: Nuf2 Antibody [NBP1-31632] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: Nuf2 Antibody [NBP1-31632]](https://resources.bio-techne.com/images/products/Nuf2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31632-img0008.jpg "Immunocytochemistry/ Immunofluorescence: Nuf2 Antibody [NBP1-31632]")
Immunocytochemistry/ Immunofluorescence: Nuf2 Antibody [NBP1-31632]
Immunocytochemistry/Immunofluorescence: Nuf2 Antibody [NBP1-31632] - NUF2 antibody detects NUF2 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NUF2 stained by NUF2 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI.![Western Blot: Nuf2 Antibody [NBP1-31632]](https://resources.bio-techne.com/images/products/Nuf2-Antibody-Western-Blot-NBP1-31632-img0007.jpg "Western Blot: Nuf2 Antibody [NBP1-31632]")
Western Blot: Nuf2 Antibody [NBP1-31632]
Western Blot: Nuf2 Antibody [NBP1-31632] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Nuf2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Nuf2
Alternate Names
cancer/testis antigen 106, CDCA1hsNuf2, cell division cycle associated 1, Cell division cycle-associated protein 1, CT106, hNuf2, kinetochore protein Nuf2, NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae), NUF2RhNuf2R
Gene Symbol
NUF2
Additional Nuf2 Products
Product Documents for Nuf2 Antibody
Product Specific Notices for Nuf2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.