Nuf2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21404

Novus Biologicals, part of Bio-Techne
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NBP3-21404-100ul
NBP3-21404-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Nuf2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YGDSVDCLPSCQLEVQLYQKKIQDLSDNREKLASILKESLNLEDQIESDESELKKLKTEENSFKRLMIVKKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nuf2 Antibody

Western Blot: Nuf2 Antibody [NBP3-21404] -

Western Blot: Nuf2 Antibody [NBP3-21404] -

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG
Immunocytochemistry/Immunofluorescence: Nuf2 Antibody [NBP3-21404] -

Immunocytochemistry/Immunofluorescence: Nuf2 Antibody [NBP3-21404] -

Staining of human cell line U-251 MG shows localization to nucleoplasm.

Applications for Nuf2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nuf2

Nuf2, also known as Kinetochore protein Nuf2, is a 54 kDa 464 amino acid protein, and it is involved in chromosome segregation, specifically as it relates to spindle checkpoint activity with the kinetochore-associated NDC80 complex. This protein is necessary for kinetochore stability and organization of microtubule binding sites on the outer plate, forming interactions with NDC80/HEC-1-CDCA1, SPBC24-SPBC25, Bub1, EXOC1, as well as AURKB/Aurora-B and CENPE in the mitotic prometaphase, DNA replication, and Cell Cycle pathways. Current research is being performed on this proteins involvement in gastric, lung, and breast cancers, in addition to essential hypertension, schizophrenia, and duodenogastric reflux.

Alternate Names

cancer/testis antigen 106, CDCA1hsNuf2, cell division cycle associated 1, Cell division cycle-associated protein 1, CT106, hNuf2, kinetochore protein Nuf2, NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae), NUF2RhNuf2R

Gene Symbol

NUF2

Additional Nuf2 Products

Product Documents for Nuf2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nuf2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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