NuMA Antibody (SPM300)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45227
![Immunohistochemistry-Paraffin: NuMA Antibody (SPM300) [NBP2-45227]](https://resources.bio-techne.com/images/products/NuMA-Antibody-SPM300-Immunohistochemistry-Paraffin-NBP2-45227-img0001.jpg "Immunohistochemistry-Paraffin: NuMA Antibody (SPM300) [NBP2-45227]")
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # SPM300
Concentration
0.2 mg/ml
Product Summary for NuMA Antibody (SPM300)
Immunogen
Colon carcinoma 174T cells
Localization
Nuclear
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Theoretical MW
228 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11547)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for NuMA Antibody (SPM300)
Immunohistochemistry-Paraffin: NuMA Antibody (SPM300) [NBP2-45227]
Immunohistochemistry-Paraffin: NuMA Antibody (SPM300) [NBP2-45227] - Human Tonsil stained with NuMA Monoclonal Antibody (SPM300)Applications for NuMA Antibody (SPM300)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
PEG purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: NuMA
Long Name
Nuclear Mitotic Apparatus Protein 1
Alternate Names
NMP-22, NUMA1, SP-H Antigen
Gene Symbol
NUMA1
UniProt
Additional NuMA Products
Product Documents for NuMA Antibody (SPM300)
Product Specific Notices for NuMA Antibody (SPM300)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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