Nup53 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92214

Novus Biologicals, part of Bio-Techne
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NBP1-92214
NBP1-92214-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Nup53 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAAFAVEPQGPALGSEPMMLGSPTSPKPGVNAQFLPGFLMGDLPAPVTPQPRSISGPSVGVMEMRSPLLAGGSPPQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nup53 Antibody

Western Blot: Nup53 Antibody [NBP1-92214]

Western Blot: Nup53 Antibody [NBP1-92214]

Western Blot: Nup53 Antibody [NBP1-92214] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: Nup53 Antibody [NBP1-92214]

Immunocytochemistry/ Immunofluorescence: Nup53 Antibody [NBP1-92214]

Immunocytochemistry/Immunofluorescence: Nup53 Antibody [NBP1-92214] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear membrane.
Immunohistochemistry-Paraffin: Nup53 Antibody [NBP1-92214]

Immunohistochemistry-Paraffin: Nup53 Antibody [NBP1-92214]

Immunohistochemistry-Paraffin: Nup53 Antibody [NBP1-92214] - Staining of human prostate shows strong positivity in nuclear membrane in glandular cells.
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Applications for Nup53 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nup53

Nup53 encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is part of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. [provided by RefSeq]

Alternate Names

35 kDa nucleoporin, Mitotic phosphoprotein 44, MP-44, MP44NUP53, NP44, Nuclear pore complex protein Nup53, nucleoporin 35kDa, Nucleoporin Nup35, nucleoporin NUP53

Gene Symbol

NUP35

Additional Nup53 Products

Product Documents for Nup53 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nup53 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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