Nup53 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24637

Novus Biologicals, part of Bio-Techne
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NBP2-24637
NBP2-24637SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Canine, Primate

Predicted:

Chicken (94%), Rat (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Nup53 Antibodies »

Product Summary for Nup53 Antibody

Immunogen

A portion of amino acids 280-326 of human NUP35 was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 89% homologous in clawed Frog.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nup53 Antibody

Western Blot: Nup53 Antibody [NBP2-24637]

Western Blot: Nup53 Antibody [NBP2-24637]

Western Blot: Nup53 Antibody [NBP2-24637] - Analysis of NUP35 in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate using NBP2-24637. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: Nup53 Antibody [NBP2-24637]

Immunohistochemistry-Paraffin: Nup53 Antibody [NBP2-24637]

Immunohistochemistry-Paraffin: Nup53 Antibody [NBP2-24637] - Analysis of NUP35 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.

Applications for Nup53 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-3 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nup53

Nup53 encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is part of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. [provided by RefSeq]

Alternate Names

35 kDa nucleoporin, Mitotic phosphoprotein 44, MP-44, MP44NUP53, NP44, Nuclear pore complex protein Nup53, nucleoporin 35kDa, Nucleoporin Nup35, nucleoporin NUP53

Gene Symbol

NUP35

UniProt

Q8NFH5 (Human)

Additional Nup53 Products

Product Documents for Nup53 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nup53 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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