NUP54 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85899

Novus Biologicals, part of Bio-Techne
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NBP1-85899
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NUP54 Antibodies »

Product Summary for NUP54 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PATTLYAHFEQANIKTQLQQLGVTLSMTRTELSPAQIKQLLQNPPAGVDPIIWEQAKVDNPDSEKLIPVPMVGFKELLRRLKVQDQMTKQHQTR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NUP54 Antibody

Western Blot: NUP54 Antibody [NBP1-85899]

Western Blot: NUP54 Antibody [NBP1-85899]

Western Blot: NUP54 Antibody [NBP1-85899] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: NUP54 Antibody [NBP1-85899]

Immunohistochemistry-Paraffin: NUP54 Antibody [NBP1-85899]

Immunohistochemistry-Paraffin: NUP54 Antibody [NBP1-85899] - Staining of human testis shows moderate to strong nuclear and nuclear membrane positivity in cells in seminiferous ducts.
Western Blot: NUP54 Antibody [NBP1-85899]

Western Blot: NUP54 Antibody [NBP1-85899]

Western Blot: NUP54 Antibody [NBP1-85899] - Analysis in human cell line HepG2.
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Applications for NUP54 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NUP54

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the phe-gly (FG) repeat-containing nucleoporin subset. [provided by RefSeq]

Alternate Names

54 kDa nucleoporin, MGC13407, nucleoporin 54kD, nucleoporin 54kDa, nucleoporin p54

Gene Symbol

NUP54

Additional NUP54 Products

Product Documents for NUP54 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NUP54 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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