NUP62 Antibody (M436)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23226
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # M436
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for NUP62 Antibody (M436)
Immunogen
Clone M436 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human nucleoporin p62. This sequence has high homology to similar regions in rat, mouse, and chicken NUP62. [UniProt# P37198]
Specificity
This antibody detects a 62 kDa protein corresponding to the apparent molecular mass of NUP62 on SDS-PAGE immunoblots of human HeLa and rat A7r5 cells. In immunocytochemistry, anti-Nucleoporin p62 specifically stains nuclei and nuclear envelope in paraformaldehyde fixed and NP-40 permeabilized cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for NUP62 Antibody (M436)
Western Blot: NUP62 Antibody (M436) [NBP3-23226] -
Western Blot: NUP62 Antibody (M436) [NBP3-23226] - Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediatefilament protein (lane 1), anti-Nucleoporin p62 NBP3-23226 (lane 2), anti-Hsp60 mitochondrial protein (lane 3), and anti-Calnexin endoplasmic reticulum protein (lane 4).
Immunocytochemistry/Immunofluorescence: NUP62 Antibody (M436) [NBP3-23226] -
Immunocytochemistry/Immunofluorescence: NUP62 Antibody (M436) [NBP3-23226] - Immunocytochemical labeling of Nucleoporin p62 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with mouse monoclonal anti-Nucleoporin p62 (N-terminal region) and the antibody was detected using Goat anti-Mouse secondary antibodies conjugated to DyLight(R) 488.Applications for NUP62 Antibody (M436)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: NUP62
Alternate Names
DKFZp547L134,62 kDa nucleoporin, FLJ20822, FLJ43869, IBSNp62, MGC841, nuclear pore glycoprotein p62, nucleoporin 62kD, nucleoporin 62kDa, SNDINucleoporin Nup62
Gene Symbol
NUP62
Additional NUP62 Products
Product Documents for NUP62 Antibody (M436)
Product Specific Notices for NUP62 Antibody (M436)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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