NUT Antibody (1G6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80886

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G6

Format

Azide and BSA Free

Concentration

1 mg/ml

View all NUT Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to an internal portion of human NUT (between amino acids 400-700) [UniProt Q86Y26].

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for NUT Antibody (1G6) - Azide and BSA Free

Western Blot: NUT Antibody (1G6)Azide and BSA Free [NBP2-80886]

Western Blot: NUT Antibody (1G6)Azide and BSA Free [NBP2-80886]

Western Blot: NUT Antibody (1G6) - Azide and BSA Free [NBP2-80886] - WB analysis of NUT/ NUTM1 protein in (A) human testis lysate and on (B) partial recombinant NUT protein (expected molecular weight ~17kda) with NUT antibody (clone 46B3D2.1G6) at a concentration of 2 ug/ml. In human testis, this antibody detected Isoform 1 and Isoform 2 at ~120 kDa and ~46kDa respectively. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: NUT Antibody (1G6) - Azide and BSA Free [NBP2-80886]

Immunocytochemistry/ Immunofluorescence: NUT Antibody (1G6) - Azide and BSA Free [NBP2-80886]

Immunocytochemistry/Immunofluorescence: NUT Antibody (1G6) - Azide and BSA Free [NBP2-80886] - NUT antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x. Image from the standard format of this antibody.
Immunohistochemistry: NUT Antibody (1G6) - Azide and BSA Free [NBP2-80886]

Immunohistochemistry: NUT Antibody (1G6) - Azide and BSA Free [NBP2-80886]

Immunohistochemistry: NUT Antibody (1G6) - Azide and BSA Free [NBP2-80886] - IHC-P analysis of NUT protein in normal human skin tissue section using NUT antibody (clone 46B3D2.1G6) at a concentration of 5 ug/ml. This antibody stained the nuclei and cytoplasm of different epidermal cell types such as granulosa cells, keratinocytes,

Applications for NUT Antibody (1G6) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

2-5 ug/ml

Immunohistochemistry-Paraffin

2-5 ug/ml

Western Blot

2 ug/ml
Application Notes
In WB, this antibody detects Isoform 1 (~120 kDa), Isoform 2 (~46kDa) and a non-specific/uncharacterized band (~100kDa). In immunostaining assays, this antibody is expected to depict the nuclear and cytoplasmic staining pattern.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NUT

NUT is known to fuse with BRD3 and BRD4 in NUT midline carcinoma (NMC), a rare and aggressive human cancer. In the majority of NMCs (~75%), most of the coding sequence is fused with BRD4 creating chimeric genes that encode BRD-NUT fusion proteins. In other cases, it fuses with BRD3 or an unknown partner gene. This tumor is often termed NUT-variant. To date, NMCs are still frequently undiagnosed or misdiagnosed and there are no effective treatment options.

Alternate Names

C15orf55, chromosome 15 open reading frame 55, DKFZp434O192, FAM22H, MGC138683, MGC138684, Nuclear protein in testis, NUT, protein NUT

Gene Symbol

NUTM1

Additional NUT Products

Product Documents for NUT Antibody (1G6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NUT Antibody (1G6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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