NUT Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32672
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within C terminal Mouse NUT. (Uniprot: Q86Y26)
Localization
Cytoplasm, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NUT Antibody
Western Blot: NUT Antibody [NBP3-32672]
Western Blot: NUT Antibody [NBP3-32672] - Western blot analysis of NUT on mouse testis tissue lysates with Rabbit anti-NUT antibody (NBP3-32672) at 1/2,000 dilution.Lysates/proteins at 40 ug/Lane.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Exposure time: 2 minutes 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32672) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: NUT Antibody [NBP3-32672]
Immunohistochemistry: NUT Antibody [NBP3-32672] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUT antibody (NBP3-32672) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32672) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry: NUT Antibody [NBP3-32672]
Immunohistochemistry: NUT Antibody [NBP3-32672] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling NUT with Rabbit anti-NUT antibody (NBP3-32672) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32672, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Applications for NUT Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500-1:800
Immunohistochemistry-Paraffin
1:1000-1:20000
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NUT
Alternate Names
C15orf55, chromosome 15 open reading frame 55, DKFZp434O192, FAM22H, MGC138683, MGC138684, Nuclear protein in testis, NUT, protein NUT
Gene Symbol
NUTM1
Additional NUT Products
Product Documents for NUT Antibody
Product Specific Notices for NUT Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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