Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NXF1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 569 and 619 of human Nuclear RNA Export Factor 1 using the numbering given in entry NP_006353.2 (GeneID 10482).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NXF1 Antibody

Western Blot: NXF1 Antibody [NBP2-22267]

Western Blot: NXF1 Antibody [NBP2-22267]

Western Blot: NXF1 Antibody [NBP2-22267] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22267 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: NXF1 Antibody [NBP2-22267]

Immunoprecipitation: NXF1 Antibody [NBP2-22267]

Immunoprecipitation: NXF1 Antibody [NBP2-22267] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-NXF1 antibody NBP2-22267 used for IP at 6 ug/mg lysate. NXF1 was also immunoprecipitated by rabbit anti-NXF1 antibodies NBP2-22265 and NBP2-22266. For blotting immunoprecipitated NXF1, NBP2-22267 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for NXF1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NXF1

NXF1 is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Alternate Names

DKFZp667O0311, Mex67, mRNA export factor TAP, nuclear RNA export factor 1, TAPMEX67, tip associating protein, Tip-associated protein, Tip-associating protein

Gene Symbol

NXF1

UniProt

NP_006353.2 (Human)

Additional NXF1 Products

Product Documents for NXF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NXF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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