NXF5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-57260

Novus Biologicals, part of Bio-Techne
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NBP1-57260
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all NXF5 Antibodies »

Product Summary for NXF5 Antibody

Immunogen

Synthetic peptides corresponding to NXF5 (nuclear RNA export factor 5) The peptide sequence was selected from the middle region of NXF5. Peptide sequence ITERNFPELLSLNLCNNKLYQLDGLSDITEKAPKVKTLNLSKNKLESAWE. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for NXF5 Antibody

Western Blot: NXF5 Antibody [NBP1-57260]

Western Blot: NXF5 Antibody [NBP1-57260]

Western Blot: NXF5 Antibody [NBP1-57260] - Lanes: Lane 1 : 7ug HeLa cystosolic extract Lane 2: 7ug HeLa nuclear extract Primary, Antibody Dilution: 1 : 100 Secondary Antibody: Anti-rabbit-HRP Secondary, Antibody Dilution: 1 : 500 Gene name: NXF5.
Immunohistochemistry-Paraffin: NXF5 Antibody [NBP1-57260]

Immunohistochemistry-Paraffin: NXF5 Antibody [NBP1-57260]

Immunohistochemistry-Paraffin: NXF5 Antibody [NBP1-57260] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: NXF5 Antibody [NBP1-57260]

Western Blot: NXF5 Antibody [NBP1-57260]

Western Blot: NXF5 Antibody [NBP1-57260] - Jurkat cell lysate, Antibody Titration: 2.5ug/ml
View 1 more images

Applications for NXF5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NXF5

NXF5 is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. Five transcript variants that encode different isoforms have been found for this gene.

Alternate Names

nuclear RNA export factor 5, TAPL1, TAPL-1, TAP-like protein 1

Gene Symbol

NXF5

UniProt

Q9H1B4 (Human)

Additional NXF5 Products

Product Documents for NXF5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NXF5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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