O-GlcNAc Transferase p110 subunit Antibody (8G7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26448

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26448-100ul
NBP3-26448-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8G7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all O-GlcNAc Transferase/OGT Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human O-GlcNAc Transferase p110 subunit [UniProt O15294]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for O-GlcNAc Transferase p110 subunit Antibody (8G7)

O-GlcNAc Transferase p110 subunit Antibody (8G7)

Immunohistochemistry: O-GlcNAc Transferase p110 subunit Antibody (8G7) [NBP3-26448] -

Immunohistochemistry: O-GlcNAc Transferase p110 subunit Antibody (8G7) [NBP3-26448] - Image of O-GlcNAc Transferase p110 subunit Antibody (8G7) diluted at 1:100 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
O-GlcNAc Transferase p110 subunit Antibody (8G7)

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (8G7) [NBP3-26448] -

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (8G7) [NBP3-26448] - Positive Western Blot detected in: NIH/3T3 whole cell lysate, HL-60 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate.
All lanes: O-GlcNAc Transferase p110 subunit Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 117, 104, 116, 75 kDa
Observed band size: 117 kDa

Applications for O-GlcNAc Transferase p110 subunit Antibody (8G7)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: O-GlcNAc Transferase/OGT

Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

Long Name

O-Linked N-Acetylglucosamine (GlcNAc) Transferase

Alternate Names

HRNT1, OGlcNAc Transferase, OGT

Gene Symbol

OGT

Additional O-GlcNAc Transferase/OGT Products

Product Documents for O-GlcNAc Transferase p110 subunit Antibody (8G7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for O-GlcNAc Transferase p110 subunit Antibody (8G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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