O-GlcNAc Transferase p110 subunit Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32791

Novus Biologicals, part of Bio-Techne
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NBP1-32791
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Porcine (100%), Xenopus (98%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all O-GlcNAc Transferase/OGT Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human O-GlcNAc Transferase p110 subunit. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (98%).

Localization

Cytoplasm (Potential), Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for O-GlcNAc Transferase p110 subunit Antibody

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Scale bar= 10um.
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Rat brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - A. 30 ug PC-12 whole cell extract 5 % SDS-PAGE O-GlcNAc transferase antibody dilution: 1:1000
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Applications for O-GlcNAc Transferase p110 subunit Antibody

Application
Recommended Usage

Flow Cytometry

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: O-GlcNAc Transferase/OGT

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

O-Linked N-Acetylglucosamine (GlcNAc) Transferase

Alternate Names

HRNT1, OGlcNAc Transferase, OGT

Gene Symbol

OGT

Additional O-GlcNAc Transferase/OGT Products

Product Documents for O-GlcNAc Transferase p110 subunit Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for O-GlcNAc Transferase p110 subunit Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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