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O-GlcNAcase/OGA/MGEA5 Antibody (1C7)

Novus Biologicals, part of Bio-Techne | Catalog # H00010724-M02

Novus Biologicals, part of Bio-Techne
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H00010724-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MGEA5 (NP_036347, 823 a.a. ~ 916 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL

Specificity

MGEA5 - meningioma expressed antigen 5 (hyaluronidase) (1C7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for O-GlcNAcase/OGA/MGEA5 Antibody (1C7)

Immunocytochemistry/ Immunofluorescence: O-GlcNAcase/OGA/MGEA5 Antibody (1C7) [H00010724-M02]

Immunocytochemistry/ Immunofluorescence: O-GlcNAcase/OGA/MGEA5 Antibody (1C7) [H00010724-M02]

Immunocytochemistry/Immunofluorescence: O-GlcNAcase/OGA/MGEA5 Antibody (1C7) [H00010724-M02] - Analysis of monoclonal antibody to MGEA5 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: O-GlcNAcase/OGA/MGEA5 Antibody (1C7) [H00010724-M02] - Detection limit for recombinant GST tagged MGEA5 is 0.3 ng/ml as a capture antibody.

Applications for O-GlcNAcase/OGA/MGEA5 Antibody (1C7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: O-GlcNAcase/OGA

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Alternate Names

GH84, HEXC3, Hexosaminidase B, MGEA5, NCOAT, OGA, OGlcNAcase

Entrez Gene IDs

10724 (Human)

Gene Symbol

OGA

Additional O-GlcNAcase/OGA Products

Product Documents for O-GlcNAcase/OGA/MGEA5 Antibody (1C7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for O-GlcNAcase/OGA/MGEA5 Antibody (1C7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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