O-GlcNAcase/OGA/MGEA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81244

Novus Biologicals, part of Bio-Techne
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NBP1-81244
NBP1-81244-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all O-GlcNAcase/OGA Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: WTGPKVVSKEIPVESIEEVSKIIKRAPVIWDNIHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for O-GlcNAcase/OGA/MGEA5 Antibody

Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody [NBP1-81244]

Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody [NBP1-81244]

Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody [NBP1-81244] - Analysis in human cell line A-431.
Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP1-81244]

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP1-81244]

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP1-81244] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP1-81244]

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP1-81244]

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody [NBP1-81244] - Staining of human testis shows strong cytoplasmic positivity in spermatogonia in seminiferus ducts.
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Applications for O-GlcNAcase/OGA/MGEA5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: O-GlcNAcase/OGA

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Alternate Names

GH84, HEXC3, Hexosaminidase B, MGEA5, NCOAT, OGA, OGlcNAcase

Gene Symbol

OGA

Additional O-GlcNAcase/OGA Products

Product Documents for O-GlcNAcase/OGA/MGEA5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for O-GlcNAcase/OGA/MGEA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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