OAT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32390

Novus Biologicals, part of Bio-Techne
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NBP1-32390
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (95%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all OAT Antibodies »

Product Summary for OAT Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human OAT. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (86%) and Xenopus laevis (84%).

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OAT Antibody

Western Blot: OAT Antibody [NBP1-32390]

Western Blot: OAT Antibody [NBP1-32390]

Western Blot: OAT Antibody [NBP1-32390] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with OAT antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: OAT Antibody [NBP1-32390]

Immunocytochemistry/ Immunofluorescence: OAT Antibody [NBP1-32390]

Immunocytochemistry/Immunofluorescence: OAT Antibody [NBP1-32390] - OAT antibody detects OAT protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: OAT protein stained by OAT antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: OAT Antibody [NBP1-32390]

Immunohistochemistry-Paraffin: OAT Antibody [NBP1-32390]

Immunohistochemistry-Paraffin: OAT Antibody [NBP1-32390] - Human gastric cancer, using OAT antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications for OAT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: OAT

OAT, also known as mitochondrial enzyme ornithine aminotransferase, is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. ( L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid.) Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Recombinant OAT protein was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

GACR, HOGA, OAT ornithine aminotransferase, OATASE, OKT

Gene Symbol

OAT

Additional OAT Products

Product Documents for OAT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OAT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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