OBFC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88744

Novus Biologicals, part of Bio-Techne
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NBP1-88744
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all OBFC1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:DTIRVRGSIRTYREEREIHATTYYKVDDPVWNIQIARMLELPTIYRKVYDQPFHSSALEKEEALSNPGALDLPSLTSLLSEKAKEFLMENRVQSF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OBFC1 Antibody

Western Blot: OBFC1 Antibody [NBP1-88744]

Western Blot: OBFC1 Antibody [NBP1-88744]

Western Blot: OBFC1 Antibody [NBP1-88744] - Analysis in control (vector only transfected HEK293T lysate) and OBFC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: OBFC1 Antibody [NBP1-88744]

Immunocytochemistry/ Immunofluorescence: OBFC1 Antibody [NBP1-88744]

Immunocytochemistry/Immunofluorescence: OBFC1 Antibody [NBP1-88744] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.

Applications for OBFC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OBFC1

OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNApolymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 (PubMed19119139)). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM613130) (Miyake et al., 2009 (PubMed 19854130)).(supplied by OMIM)

Alternate Names

AAF44, AAF-44, alpha accessory factor 44, bA541N10.2, CST complex subunit STN1, oligonucleotide/oligosaccharide-binding fold containing 1, Oligonucleotide/oligosaccharide-binding fold-containing protein 1, replication protein A 32 kDa subunit, RP11-541N10.2, RPA-32, STN1FLJ22559, Suppressor of cdc thirteen homolog

Gene Symbol

STN1

Additional OBFC1 Products

Product Documents for OBFC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for OBFC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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