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OBFC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99011

Novus Biologicals, part of Bio-Techne
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NBP2-99011-100ul
NBP2-99011-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for OBFC1 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human OBFC1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for OBFC1 Antibody

Immunocytochemistry/ Immunofluorescence: OBFC1 Antibody [NBP2-99011]

Immunocytochemistry/ Immunofluorescence: OBFC1 Antibody [NBP2-99011]

Immunocytochemistry/Immunofluorescence: OBFC1 Antibody [NBP2-99011] - Immunofluorescence staining of OBFC1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human OBFC1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Immunohistochemistry-Paraffin: OBFC1 Antibody [NBP2-99011]

Immunohistochemistry-Paraffin: OBFC1 Antibody [NBP2-99011]

Immunohistochemistry-Paraffin: OBFC1 Antibody [NBP2-99011] - Immunochemical staining of human OBFC1 in human vagina with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Applications for OBFC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OBFC1

OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNApolymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 (PubMed19119139)). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM613130) (Miyake et al., 2009 (PubMed 19854130)).(supplied by OMIM)

Alternate Names

AAF44, AAF-44, alpha accessory factor 44, bA541N10.2, CST complex subunit STN1, oligonucleotide/oligosaccharide-binding fold containing 1, Oligonucleotide/oligosaccharide-binding fold-containing protein 1, replication protein A 32 kDa subunit, RP11-541N10.2, RPA-32, STN1FLJ22559, Suppressor of cdc thirteen homolog

Gene Symbol

STN1

Additional OBFC1 Products

Product Documents for OBFC1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OBFC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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