Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all OBFC2B Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 161 and 211 of human single-stranded DNA-binding protein 1 using the numbering given in entry NP_076973.1 (GeneID 79035).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OBFC2B Antibody

Western Blot: OBFC2B Antibody [NBP1-06561]

Western Blot: OBFC2B Antibody [NBP1-06561]

Western Blot: OBFC2B Antibody [NBP1-06561] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB) and 293T (T; 50 ug for WB; 1 mg for IP, 20% of IP loaded) cells. NBP1-06561 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
Immunohistochemistry: OBFC2B Antibody [NBP1-06561]

Immunohistochemistry: OBFC2B Antibody [NBP1-06561]

Immunohistochemistry: OBFC2B Antibody [NBP1-06561] - Sample: FFPE sections of human colon carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- SSB1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for OBFC2B Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 mcg/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for human tissues. Epitope retrieval with citrate buffer pH6.0 is recommended for mouse tissues

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: OBFC2B

Single-stranded DNA (ssDNA)-binding proteins, such as OBFC2B, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage (Richard et al., 2008 [PubMed 18449195]).[supplied by OMIM]

Alternate Names

LP3587, MGC2731, oligonucleotide/oligosaccharide-binding fold containing 2B, Oligonucleotide/oligosaccharide-binding fold-containing protein 2B, Sensor of single-strand DNA complex subunit B1, Sensor of ssDNA subunit B1, Single-stranded DNA-binding protein 1, SOSS complex subunit B1, SOSS-B1, SSB1hSSB1

Entrez Gene IDs

79035 (Human)

Gene Symbol

NABP2

UniProt

NP_076973.1 (Human)

Additional OBFC2B Products

Product Documents for OBFC2B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OBFC2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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