Oct-4B Antibody (OTI7H1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45376
![Immunofluorescence: Oct-4B Antibody (OTI7H1) [NBP2-45376]](https://resources.bio-techne.com/images/products/Oct-4B-Antibody-OTI7H1-Immunofluorescence-NBP2-45376-img0002.jpg "Immunofluorescence: Oct-4B Antibody (OTI7H1) [NBP2-45376]")
![Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376]](https://resources.bio-techne.com/images/products/Oct-4B-Antibody-OTI7H1-Western-Blot-NBP2-45376-img0003.jpg "Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI7H1
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human POU5F1(NP_002692) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
38.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Oct-4B Antibody (OTI7H1)
Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376]
Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU5F1P1 .![Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376]](https://resources.bio-techne.com/images/products/Oct-4B-Antibody-OTI7H1-Western-Blot-NBP2-45376-img0004.jpg "Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376]")
Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376]
Western Blot: Oct-4B Antibody (OTI7H1) [NBP2-45376] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).![Immunofluorescence: Oct-4B Antibody (OTI7H1) [NBP2-45376]](https://resources.bio-techne.com/images/products/Oct-4B-Antibody-OTI7H1-Immunofluorescence-NBP2-45376-img0001.jpg "Immunofluorescence: Oct-4B Antibody (OTI7H1) [NBP2-45376]")
Immunofluorescence: Oct-4B Antibody (OTI7H1) [NBP2-45376]
Immunofluorescence: Oct-4B Antibody (OTI7H1) [NBP2-45376] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY POU5F1P1.Applications for Oct-4B Antibody (OTI7H1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:200 - 1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Oct-4B
Long Name
Octamer-binding Transcription Factor 4B
Alternate Names
OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1
Gene Symbol
POU5F1
Additional Oct-4B Products
Product Documents for Oct-4B Antibody (OTI7H1)
Product Specific Notices for Oct-4B Antibody (OTI7H1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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