OCT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24677

Novus Biologicals, part of Bio-Techne
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NBP2-24677
NBP2-24677SS
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Key Product Details

Species Reactivity

Validated:

Human, Canine, Equine, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all OCT1 Antibodies »

Product Summary for OCT1 Antibody

Immunogen

A portion of amino acids 1-50 of human OCT-1 was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 83% homologous in mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for OCT1 Antibody

Western Blot: OCT1 Antibody [NBP2-24677]

Western Blot: OCT1 Antibody [NBP2-24677]

Western Blot: OCT1 Antibody [NBP2-24677] - Western blot analysis of OCT-1 in human HL60 cell lysate using NBP2-24677 at 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: OCT1 Antibody [NBP2-24677]

Immunocytochemistry/ Immunofluorescence: OCT1 Antibody [NBP2-24677]

Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP2-24677] - NTERA2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-OCT1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-24677]

Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-24677]

Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-24677] - Analysis of OCT-1 in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Applications for OCT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

3-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OCT1

Oct1 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. It modulates transcription transactivation by NR3C1, AR and PGR and interacts with NR3C1, AR and PGR. Oct1 is Ubiquitous and belongs to the POU transcription factor family class 2 subfamily. Oct1 contains 1 homeobox domain.

Long Name

Octamer-binding Transcription Factor 1

Alternate Names

FLJ42836, NF-A1, Oct-1, OCT1class 2, transcription factor 1, OTF1, POU class 2 homeobox 1, POU domain class 2, transcription factor 1, POU2F1

Gene Symbol

POU2F1

Additional OCT1 Products

Product Documents for OCT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for OCT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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