OCT6 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13349
![Immunohistochemistry-Paraffin: OCT6 Antibody [NBP3-13349]](https://resources.bio-techne.com/images/products/OCT6-Antibody-Immunohistochemistry-Paraffin-NBP3-13349-img0002.jpg "Immunohistochemistry-Paraffin: OCT6 Antibody [NBP3-13349]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for OCT6 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of mouse OCT6. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for OCT6 Antibody
![Western Blot: OCT6 Antibody [NBP3-13349]](https://resources.bio-techne.com/images/products/OCT6-Antibody-Western-Blot-NBP3-13349-img0001.jpg "Western Blot: OCT6 Antibody [NBP3-13349]")
Western Blot: OCT6 Antibody [NBP3-13349]
Western Blot: OCT6 Antibody [NBP3-13349] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with OCT6 antibody (NBP3-13349) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.![Immunocytochemistry/ Immunofluorescence: OCT6 Antibody [NBP3-13349]](https://resources.bio-techne.com/images/products/OCT6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13349-img0003.jpg "Immunocytochemistry/ Immunofluorescence: OCT6 Antibody [NBP3-13349]")
Immunocytochemistry/ Immunofluorescence: OCT6 Antibody [NBP3-13349]
Immunocytochemistry/Immunofluorescence: OCT6 Antibody [NBP3-13349] - OCT6 antibody detects OCT6 protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT6 stained by OCT6 antibody (NBP3-13349) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.![Immunohistochemistry-Paraffin: OCT6 Antibody [NBP3-13349]](https://resources.bio-techne.com/images/products/OCT6-Antibody-Immunohistochemistry-Paraffin-NBP3-13349-img0005.jpg "Immunohistochemistry-Paraffin: OCT6 Antibody [NBP3-13349]")
Immunohistochemistry-Paraffin: OCT6 Antibody [NBP3-13349]
Immunohistochemistry-Paraffin: OCT6 Antibody [NBP3-13349] - OCT6 antibody detects OCT6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E10.5 embryo. Green: OCT6 stained by OCT6 antibody (NBP3-13349) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Immunohistochemistry-Frozen: OCT6 Antibody [NBP3-13349]](https://resources.bio-techne.com/images/products/OCT6-Antibody-Immunohistochemistry-Frozen-NBP3-13349-img0004.jpg "Immunohistochemistry-Frozen: OCT6 Antibody [NBP3-13349]")
Applications for OCT6 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: OCT6
Alternate Names
Oct-6, OCT6POU domain transcription factor SCIP, Octamer-binding protein 6, Octamer-binding transcription factor 6, OTF6, OTF-6, POU class 3 homeobox 1, POU domain class 3, transcription factor 1, POU domain, class 3, transcription factor 1, SCIP
Gene Symbol
POU3F1
Additional OCT6 Products
Product Specific Notices for OCT6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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