ODF2 [p Ser796] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB110-85525
Key Product Details
Species Reactivity
Human, Chimpanzee, Rhesus Macaque
Applications
Dot Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified ODF2 [p Ser796] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near S796 of human ODF2. (Uniprot: Q5BJF6)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with ODF2 from human, chimpanzee and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with ODF2from other sources has not been determined.
Modification
p Ser796
Specificity
This antibody is specific for human ODF2 protein phosphorylated at S796. A BLAST analysis was used to suggest cross-reactivity with ODF2 from human, chimpanzee and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with ODF2from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for ODF2 [p Ser796] Antibody
Western Blot: ODF2 [p Ser796] Antibody [NB110-85525] - Western Blot of ODF2 [p Ser796] antibody. Lane 1: MW.Lane 2: Human Semen Lysate.Lane 3: MCF7 WCL. Lane 4: Molt 4 WCL. Lane 5: HeLa WCL. Load: 10ug per lane. Blocking:. Primary Antibody: Anti-ODF2 pS796 at 1ug/mL overnight at 2-8C.Secondary Antibody: Goat anti-Rabbit HRP 1:40,000 diluted in for 30 minutes at RT.Expect: 93kDa.
ELISA: ODF2 [p Ser796] Antibody [NB110-85525] - ELISA of ODF2 [p Ser796] antibody. Antigen: BSA conjugates of ODF2 pS796 (purple) and S796 (blue).Coating amount: 0.1 ug per well. Primary antibody: ODF2 at 5 ug/mL. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody at 1:8,500. Substrate: TMB.
Applications for ODF2 [p Ser796] Antibody
Application
Recommended Usage
ELISA
1:2000-1:10000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:200-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 93 kDa in size corresponding to phosphorylated Cenexin-1 by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human Cenexin-1 pS796 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: ODF2
Alternate Names
Cenexin, FLJ44866, MGC111096, MGC9034, ODF2/1, ODF2/2, ODF8484-kD, outer dense fiber of sperm tails 2, outer dense fiber protein 2, sperm tail structural protein
Entrez Gene IDs
4957 (Human)
Gene Symbol
ODF2
UniProt
Additional ODF2 Products
Product Documents for ODF2 [p Ser796] Antibody
Product Specific Notices for ODF2 [p Ser796] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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