Opsin 1 (Medium Wave) Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB110-74730
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A synthetic peptide from mouse Opsin 1 (Medium Wave) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:33803057).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Opsin 1 (Medium Wave) Antibody
Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730]
Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Protection of the retina against light damage in Grk1-/- mice subjected to BLE (12.5 klux for 0.5 hours) 1 week prior to imaging. Drug combination (M+B+T) or vehicle was injected i.p. 0.5 hours prior to induction of light damage (replicates are presented in Table 2). Representative flat-mount retina image of typical M- (green) and S-cone (blue) populations in vehicle-treated mouse retinas 1 week after BLE. Dashed white lines show border between damaged and healthy site in cone population. M+B+T at a dose 10-1-0.5 mg/kg maintained cone population at ~95%, whereas ONL thickness (representative of rod population) with this dose was decreased by ~50%. M+B+T 50-5-2.5 mg/kg dose led to full protection in both rod and cone population. Image collected and cropped by CiteAb from the following publication (https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.19-26560) licensed under a CC-BY license.Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730]
Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IF analysis of Opsin 1 in paraffin embedded and frozen mouse retina tissues. Image courtesy of product review submitted by Linda Vuong.Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730]
Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Retinal whole mount of BALB/c mouse stained against anti M-opsin secondary (AF647) antibody and peanut agglutinin (Fluorescein). This image was submitted via customer Review.Applications for Opsin 1 (Medium Wave) Antibody
Application
Recommended Usage
Immunohistochemistry
1:250-1:500
Immunohistochemistry-Frozen
1:250-1:500
Immunohistochemistry-Paraffin
1:250-1:500
Western Blot
1:250-1:500
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30307502). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29162627).
Reviewed Applications
Read 3 reviews rated 4.7 using NB110-74730 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Reconstitution
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.
Formulation
Lyophilized from whole antisera
Preservative
0.02% Benzalkonium chloride
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Opsin 1 (Medium Wave)
Alternate Names
GCP, GOP, Green cone photoreceptor pigment, Green-sensitive opsin, medium-wave-sensitive opsin 1, OPN1MW, opsin 1 (cone pigments), medium-wave-sensitive 2
Gene Symbol
OPN1MW
UniProt
Additional Opsin 1 (Medium Wave) Products
Product Documents for Opsin 1 (Medium Wave) Antibody
Product Specific Notices for Opsin 1 (Medium Wave) Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...