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Opsin 1 (Medium Wave) Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-74730

Novus Biologicals, part of Bio-Techne
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NB110-74730-0.025ml
NB110-74730

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

A synthetic peptide from mouse Opsin 1 (Medium Wave) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33803057).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Opsin 1 (Medium Wave) Antibody

Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730]

Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730]

Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Protection of the retina against light damage in Grk1-/- mice subjected to BLE (12.5 klux for 0.5 hours) 1 week prior to imaging. Drug combination (M+B+T) or vehicle was injected i.p. 0.5 hours prior to induction of light damage (replicates are presented in Table 2). Representative flat-mount retina image of typical M- (green) and S-cone (blue) populations in vehicle-treated mouse retinas 1 week after BLE. Dashed white lines show border between damaged and healthy site in cone population. M+B+T at a dose 10-1-0.5 mg/kg maintained cone population at ~95%, whereas ONL thickness (representative of rod population) with this dose was decreased by ~50%. M+B+T 50-5-2.5 mg/kg dose led to full protection in both rod and cone population. Image collected and cropped by CiteAb from the following publication (https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.19-26560) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730]

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730]

Immunohistochemistry-Paraffin: Opsin 1 (Medium Wave) Antibody [NB110-74730] - IF analysis of Opsin 1 in paraffin embedded and frozen mouse retina tissues. Image courtesy of product review submitted by Linda Vuong.
Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730]

Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730]

Immunohistochemistry: Opsin 1 (Medium Wave) Antibody [NB110-74730] - Retinal whole mount of BALB/c mouse stained against anti M-opsin secondary (AF647) antibody and peanut agglutinin (Fluorescein). This image was submitted via customer Review.

Applications for Opsin 1 (Medium Wave) Antibody

Application
Recommended Usage

Immunohistochemistry

1:250-1:500

Immunohistochemistry-Frozen

1:250-1:500

Immunohistochemistry-Paraffin

1:250-1:500

Western Blot

1:250-1:500
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30307502). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29162627).

Reviewed Applications

Read 3 reviews rated 4.7 using NB110-74730 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

0.02% Benzalkonium chloride

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Opsin 1 (Medium Wave)

FUNCTION: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in cone photoreceptor cells.

Alternate Names

GCP, GOP, Green cone photoreceptor pigment, Green-sensitive opsin, medium-wave-sensitive opsin 1, OPN1MW, opsin 1 (cone pigments), medium-wave-sensitive 2

Entrez Gene IDs

14539 (Mouse); 89810 (Rat)

Gene Symbol

OPN1MW

UniProt

Additional Opsin 1 (Medium Wave) Products

Product Documents for Opsin 1 (Medium Wave) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Opsin 1 (Medium Wave) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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