Optineurin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84683

Novus Biologicals, part of Bio-Techne
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NBP1-84683
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Optineurin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Optineurin Antibody

Western Blot: Optineurin Antibody [NBP1-84683]

Western Blot: Optineurin Antibody [NBP1-84683]

Western Blot: Optineurin Antibody [NBP1-84683] - Analysis using Anti-OPTN antibody NBP1-84683 (A) shows similar pattern to independent antibody NBP1-84682 (B).
Immunocytochemistry/ Immunofluorescence: Optineurin Antibody [NBP1-84683]

Immunocytochemistry/ Immunofluorescence: Optineurin Antibody [NBP1-84683]

Immunocytochemistry/Immunofluorescence: Optineurin Antibody [NBP1-84683] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84683]

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84683]

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84683] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
View 5 more images

Applications for Optineurin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Optineurin

Optineurin encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Alternate Names

ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, OPTN

Gene Symbol

OPTN

Additional Optineurin Products

Product Documents for Optineurin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Optineurin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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