Optineurin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26700

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Optineurin Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to an internal portion of human Optineurin between amino acids 150-350 [UniProt Q96CV9].

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Optineurin Antibody

Optineurin Antibody Immunohistochemical Staining of Optineurin in Human Colon

Immunohistochemical Staining of Optineurin in Human Colon

Analysis of a FFPE tissue section of human colon using 1:200 dilution of Optineurin antibody (NBP3-26700). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Optineurin Antibody Immunocytochemistry Staining of Optineurin in U-87 MG Human Cell Line

Immunocytochemistry Staining of Optineurin in U-87 MG Human Cell Line

Optineurin was detected in immersion fixed U-87 MG human glioblastoma cell line using Rabbit anti-Optineurin Antigen Affinity-purified Polyclonal Antibody (Catalog # NBP3-26700) at 1 µg/mL overnight at 4C. Cells were stained using DyLight 488-conjugated Anti-Rabbit IgG (H+L) Cross-Absorbed Secondary Antibody (green) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for Optineurin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1.0 ug/ml

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Optineurin

OPTN is the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.

Alternate Names

ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, OPTN

Gene Symbol

OPTN

UniProt

Q96CV9 (Human)

Additional Optineurin Products

Product Documents for Optineurin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Optineurin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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