Orai1 Antibody (3F6H5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-75522

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In-situ Hybridization, Proximity Ligation Assay, Western Blot

Cited:

Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In Situ Hybridization, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3F6H5

Format

BSA Free

Concentration

1 mg/ml

View all Orai1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

ORAI1 antibody is predicted to have no cross reactivity to other members in the ORAI family.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Orai1 Antibody (3F6H5) - BSA Free

Western Blot: Orai1 Antibody (3F6H5)BSA Free [NBP1-75522]

Western Blot: Orai1 Antibody (3F6H5)BSA Free [NBP1-75522]

Western Blot: Orai1 Antibody (3F6H5) [NBP1-75522] - analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry-Paraffin: Orai1 Antibody (3F6H5) - BSA Free [NBP1-75522]

Immunohistochemistry-Paraffin: Orai1 Antibody (3F6H5) - BSA Free [NBP1-75522]

Immunohistochemistry-Paraffin: Orai1 Antibody (3F6H5) [NBP1-75522] - Human spleen tissue with ORAI1 antibody at 5 ug/mL.
Immunohistochemistry-Paraffin: Orai1 Antibody (3F6H5) - BSA Free [NBP1-75522]

Immunohistochemistry-Paraffin: Orai1 Antibody (3F6H5) - BSA Free [NBP1-75522]

Immunohistochemistry-Paraffin: Orai1 Antibody (3F6H5) [NBP1-75522] - ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 ug/mL
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Applications for Orai1 Antibody (3F6H5) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Use in In-situ Hybridization reported in scientific literature (PMID: 27535226). Use in Proximity ligation assay reported in scientific literature (PMID: 30018568). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:32199855).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: Orai1

Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Long Name

ORAI Calcium Release-activated Calcium Modulator 1

Alternate Names

CRACM1, ORAT1, TMEM142A

Gene Symbol

ORAI1

Additional Orai1 Products

Product Documents for Orai1 Antibody (3F6H5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Orai1 Antibody (3F6H5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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