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ORC3L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-796

Novus Biologicals, part of Bio-Techne
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NB100-796

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence QKTDHVARLTWGGC corresponding to C-Terminus according to NP_862820.1, NP_036513.2.

Specificity

This antibody is expected to recognize both reported isoforms (NP_862820.1 and NP_036513.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ORC3L Antibody

Western Blot: ORC3L Antibody [NB100-796]

Western Blot: ORC3L Antibody [NB100-796]

Western Blot: ORC3L Antibody [NB100-796] - Staining of HeLa lysate (RIPA buffer, 1.4E5 cells per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ORC3L Antibody [NB100-796]

Immunohistochemistry-Paraffin: ORC3L Antibody [NB100-796]

Immunohistochemistry-Paraffin: ORC3L Antibody [NB100-796] - Staining of paraffin embedded Human Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ORC3L Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 75 kDa band observed in lysates of HeLa (calculated MW of 82.3 kDa band according to NP_862820.1 and NP_036513.2). An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC-P: Paraffin embedded Human Brain (Cortex)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ORC3L

ORC3L is involved in DNA replication origin binding.

Alternate Names

IMAGE50150, LAT, LATHEOorigin recognition complex, subunit 3-like (yeast), ORC3Lhomolog of latheo, Drosophila, origin recognition complex subunit 3, Origin recognition complex subunit Latheo, origin recognition complex, subunit 3, origin recognition complex, subunit 3 (yeast homolog)-like, origin recognition complex, subunit 3 honolog, origin recognition complex, subunit 3 honolog (yeast), origin recognition complex, subunit 3-like

Entrez Gene IDs

23595 (Human)

Gene Symbol

ORC3

Additional ORC3L Products

Product Documents for ORC3L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ORC3L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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