Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ORC6L Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 200 and 230 of human origin recognition complex subunit 6 using the numbering given in entry NP_055136.1 (GeneID 23594).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ORC6L Antibody

Immunoprecipitation: ORC6L Antibody [NB100-74619]

Immunoprecipitation: ORC6L Antibody [NB100-74619]

Immunoprecipitation: ORC6L Antibody [NB100-74619] - Detection of Human ORC6 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ORC6 antibody NB100-74619 used for IP at 3 mcg/mg lysate. ORC6 was also immunoprecipitated by rabbit anti-ORC6 antibody NB100-74620, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ORC6L Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500
Application Notes
This antibody is not recommended for Western Blot.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ORC6L

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown that this protein and and ORC1L are loosely associated with the core complex consisting of ORC2L, -3L, -4L and -5L. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis.

Alternate Names

ORC6Lorigin recognition complex, subunit 6 homolog-like (yeast), origin recognition complex subunit 6, origin recognition complex, subunit 6, origin recognition complex, subunit 6 like (yeast)

Entrez Gene IDs

23594 (Human)

Gene Symbol

ORC6

UniProt

NP_055136.1 (Human)

Additional ORC6L Products

Product Documents for ORC6L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ORC6L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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