Orexin A/Hypocretin-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65204

Novus Biologicals, part of Bio-Techne
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NB100-65204
NB100-65204-0.1ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Concentration

5.0 mg/ml

View all Orexin A/Hypocretin-1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide, PLPDCCRQKTCSC, corresponding to amino acids 2-14 of human orexin A conjugated to KLH

Epitope

Peptide sequence: PLPDCCRQKTCSC

Reactivity Notes

Predicted cross-reactivities: Chimpanzee, Bovine, Equine, Dog, Rabbit, Porcine, Mouse, Rat, Rhesus Monkey

Localization

Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses.

Specificity

NB100-65204 is specific for human orexin A.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Orexin A/Hypocretin-1 Antibody

Immunohistochemistry-Paraffin: Orexin A/Hypocretin-1 Antibody - BSA Free [NB100-65204]

Immunohistochemistry-Paraffin: Orexin A/Hypocretin-1 Antibody - BSA Free [NB100-65204]

Immunohistochemistry-Paraffin: Orexin A/Hypocretin-1 Antibody [NB100-65204] - Staining of paraffin embedded human hypothalamus with Sheep anti Human Orexin A.

Applications for Orexin A/Hypocretin-1 Antibody

Application
Recommended Usage

ELISA

1:200-1:800

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

5-10 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

5.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Orexin A/Hypocretin-1

Orexin A (hypocretin 1) a 33 amino acid peptide and orexin B(hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Orexin A and orexin B bind to and activate two closely related G protein coupled receptors (GPCRs), termed Orexin 1 (OX1) and Orexin 2 (OX2) receptors. Dysfunction of the orexin peptide system has been linked to narcolepsy.

Alternate Names

HCRT, Hcrt1, Hypocretin-1, Hypocretin1

Entrez Gene IDs

3060 (Human)

Gene Symbol

HCRT

UniProt

O43612

Additional Orexin A/Hypocretin-1 Products

Product Documents for Orexin A/Hypocretin-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Orexin A/Hypocretin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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